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. 2019 Mar;14(3):501–505. doi: 10.4103/1673-5374.245476

Figure 3.

Figure 3

Immunofluorescence staining of late-stage neurons in the AMP group.

(A) Expression of neuron-specific Map2 and NeuN in the converted cells was detected by day 21 in the AMP group. (B) NCAM, another neuron-specific marker (Fiszbein et al., 2015) was detected in the converted cells on day 29. AMP: Ascl1 + miR124 + shP53; Tuj-1: Class III b-tubulin; Map2: microtubule-associated protein 2; NeuN: neuron-specific nuclear protein; NCAM: neural cell adhesion molecule; DAPI: 4′,6-diamidino-2-phenylindole. Scale bars: 50 μm.