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. 2019 Jan 9;6:206. doi: 10.3389/fbioe.2018.00206

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Copyright © 2019 Opdensteinen, Clodt, Müschen, Filiz and Buyel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Separation of tobacco HCPs by UF/DF. (A) Native PAGE of clarified tobacco extract and UF/DF samples using a 100 kDa RC membrane (pH 7.6, 50 mS cm⁻¹). The black arrow indicates RuBisCO (~560 kDa). (B) LDS-PAGE analysis of the protein composition of UF/DF samples (pH 7.0 and 50 mS cm⁻¹) after separation using different membranes. (C) Response surface of TSP recovery in the UF/DF permeate of a 100 kDa RC membrane relative to the load, showing dependence on pH and conductivity. (D) The pI-dependent distribution of tobacco HCPs and the corresponding mass fractions in plant extracts (Buyel et al., 2013). The bin width was 0.5. The permeate TSP recoveries were predicted by the model displayed in (C) for 50 mS cm⁻¹. MWCO, molecular weight cut-off; PESU, polyether sulfone; RC, regenerated cellulose; PSBC, polystyrene block copolymer; L, load; R, retentate; P, permeate.