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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: J Autoimmun. 2007 Nov 26;30(4):238–245. doi: 10.1016/j.jaut.2007.10.003

Fig. 2.

Fig. 2.

Immunoblot detection of iC3b attachment. Apoptotic HSG cells were incubated with media supplemented with 25% human serum (HS) from individuals with PBC or controls for 1h at 37C. After washing, cells were lysed and immunoblotted with anti-iC3b antibody followed by ECL development. (A) A representative immunoblot image is shown. (B) The mean densitometry of the iC3b bands demonstrated no statistically significant difference between iC3b attachment to apoptotic cells following incubation with PBC or control (Con) serum (p > 0.050). Student’s t test was used for the comparison.