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. Author manuscript; available in PMC: 2019 Sep 20.
Published in final edited form as: Cell Chem Biol. 2018 Jun 28;25(9):1086–1094.e7. doi: 10.1016/j.chembiol.2018.05.015

Figure 2:

Figure 2:

Inhibition of Dicer processing of pre-miR-21 in vitro and in cells. A, secondary structure of the miR-21 hairpin precursor. B, representative gel image of the inhibition of Dicer processing of pre-miR-21 by 3. C, effect of 1, 2, and 3 on mature levels in triple negative breast cancer cell line MDA-MB-231. D, effect of 1, 2, and 3 on pre-miR-21 levels in triple negative breast cancer cell line MDA-MB-231. E, effect of 3, an LNA oligonucleotide targeting miR-21 (LNA-21; Exiqon, 4100689–101) and a control LNA (Scramble; Exiqon, 199006–102) on PTEN using a luciferase reporter assay. * indicates p<0.5; ** indicates p<0.01, as determined by a two-tailed Student t test. Data represents mean ± S.E.M. of biological triplicates. See also Figures S2 and S3.