Skip to main content
. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Nat Biotechnol. 2008 Jun 22;26(7):795–797. doi: 10.1038/nbt1418

Figure 1. Chemicals that promote reprogramming efficiency.

Figure 1.

(a) The percentages of GFP+ cells induced in 4-factor (Oct4, Sox2, Klf4 and c-Myc) infected Oct4-GFP/+ MEFs treated with chemicals. Chemical treatments were: 5’-azaC (2 μM), dexamethasone (dex, 1 μM), 5’-azaC and dexamethasone, VPA (2 mM), SAHA (5 μM) and TSA (20 nM). The controls were infected MEFs without chemical treatment or treated with DMSO (the solvent for dexamethasone, SAHA and TSA). The y axis is truncated to accommodate the high percentage from the VPA treatment. For all figures in this study, standard deviations are indicated by error bars, and P values by two-tailed student t-test smaller than 0.05, 0.01 and 0.001 are indicated by one, two and three asterisks respectively. (b) Representative FACS plots from 4-factor infected MEFs treated with 5’-azaC and VPA compared to the control infected MEFs without treatment. (c) MEFs infected with the 3 factors (Oct4, Sox2, Klf4, but not c-Myc) were treated with 5’-azaC or VPA for a week and the percentage of Oct4-GFP+ cells induced was measured by FACS analysis at 10 days post-infection, and compared to 3-factor infected MEFs without chemical treatment. (d) Representative pictures at 16 days post-infection in 3-factor infected MEFs with VPA treatment compared to the control infected MEFs without VPA treatment.