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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Nat Biotechnol. 2008 Jun 22;26(7):795–797. doi: 10.1038/nbt1418

Figure 2. c-Myc-free iPS cells induced by VPA treatment resemble ES cells in gene expression and pluripotency.

Figure 2.

(a) iPS colonies exhibited typical ES cell morphology and expressed Oct4-GFP homogeneously. (b) iPS colonies exhibited high alkaline phosphatase activities. (c) Scatter plots comparing global gene expression patterns between iPS cells and ES cells, and iPS cells and MEFs. Red lines indicate the linear equivalent and two fold changes in gene expression levels between the samples. (d) Hematoxylin and eosin staining of teratoma sections showed differentiation of iPS cells to various tissues. (e) lacZ staining of a midgestation chimeric embryo from donor iPS cells carrying the Rosa26-lacZ allele, compared to the non-injected control. (f) Sections of chimeric embryos showed contribution of donor iPS cells to tissues derived from all three germ layers, including the neural tube (nt, ectoderm derivative), gut endoderm (g) and limb bud (lb, mesoderm derivative). (g) Shown here is a lacZ positive e8.5 embryo with a littermate control on the left from a mating between a wild type female and a chimera from blastocyst injection of iPS cells. Both embryos have yolk sacs attached, and are oriented with the anterior to the left.