Table 3.
Observed diversity and estimated sample coverage for bacterial and archaeal 16S rRNA amplification from DNA extracted from biodigesters during the anaerobic process.
| Sample∗ | Bacteria |
Archaea |
||||
|---|---|---|---|---|---|---|
| No. OTUs | Shannon indexa | Good’s coverage (%) | No. OTUs | Shannon indexa | Good’s coverage (%) | |
| Mix_T0 | 18211.40 | 6.39D | 78.1 | – | – | – |
| MixI_T0 | 20739.20 | 7.70C | 75.5 | 6522.00 | 5.27A | 87.7 |
| B1_T7 | 20132.20 | 8.51B | 77.1 | 6661.00 | 5.03A | 87.4 |
| B2_T7 | 20287.00 | 8.76B | 76.8 | 6264.00 | 5.51A | 88.4 |
| B1_T14 | 21732.70 | 9.66A | 76.3 | 4931.00 | 5.54A | 90.9 |
| B2_T14 | 22777.20 | 9.80A | 74.9 | 6658.00 | 5.69A | 87.5 |
| B1_T30 | 22352.40 | 9.66A | 75.2 | 6215.00 | 2.82B | 88.0 |
| B2_T30 | 21285.30 | 9.65A | 76.8 | 6422.00 | 2.79B | 87.6 |
The entire data set was rarefied to 77670 reads or 47510 reads per sample, for bacteria and archaea respectively, before alpha-diversity was calculated. aDifferent letters after Shannon index values indicate significant differences (p < 0.05). ∗Mix_T0, mixture of cheese whey and buttermilk at day 0 of incubation; MixI_T0, mixture of cheese whey and buttermilk inoculated at day 0 of incubation; B1_T7, sample collected from B1 after 7 days of incubation; B2_T7, sample collected from B2 after 7 days of incubation; B1_T14, sample collected from B1 after 14 days of incubation; B2_T14, sample collected from B2 after 14 days of incubation; B1_T30, sample collected from B1 after 30 days of incubation; B2_T30, sample collected from B2 after 30 days of incubation.