Fig. 1. Illustration of the detection of a 102 nt ssDNA (ssDNA_102) using an AeL nanopore in M+Cl– (M+ = Li+ or K+). Top: the electrostatic attraction in LiCl and the relative electrostatic repulsion in KCl of a single ssDNA_102 molecule into AeL. The illustration is not to scale. Middle: raw current traces of ssDNA_102. More frequent blockade events were observed clearly in LiCl compared with KCl from the real-time current traces. Bottom: voltage dependence of ssDNA_102 translocation. Data in this work were obtained in 1 M M+Cl– (M+ = Li+ or K+), 1 mM EDTA, 10 mM Tris buffer, and pH 8.0 with the presence of 2.0 μM ssDNA at the cis side of the AeL nanopore.
