Fig. 5.
a Effects of butyrate on amiloride-sensitive ISC (ISC-amil/sen) without HCO3− (substituted for NaCl with O2 bubbling). No significant differences were observed in ISC-amil/sen between control and 30 mM butyrate without HCO3−. Under HCO3−-free conditions, the epithelium did not have the endurance required for a long-term experiment with the third amiloride application; therefore, this comparison was performed on the second amiloride application. Values are the means ± SE (n = 8). b Effects of butyrate on the shift in amiloride-insensitive ISC (ISC-amil/insen shift). No significant differences were observed in ISC-amil/insen shift with or without HCO3− (substituted for NaCl with O2 bubbling). c Ibuprofen (Ibu; 300 μM) did not affect ISC after the application of amiloride with butyrate. d Effects of 30 mM butyrate applied to the apical side on short-circuit currents (ISC) in rat rectal colonic mucosa. e Effects of 30 mM butyrate applied to the basolateral side on ISC. f Averaged ISC-amil/insen shift by the application of butyrate to the apical, basolateral, or both sides. Values are the means ± SE. Numbers in parentheses refer to the number of experiments