Fig. 7.

RT-PCR analysis of α- and β-subunits of KCNQ channels from the mucosa of rectal colon (RC), distal colon (DC), and ileum (IL). The primers used gave the expected fragment lengths for Kcnq1 (613 bp; a), Kcnq2 (510 bp; b), Kcnq3 (790 bp; c), Kcnq4 (664 bp; d), Kcnq5 (502 bp; e), Kcne1 (281 bp; f), Kcne2 (224 bp; g), Kcne3 (232 bp; h), Kcne4 (476 bp; i), and Kcne5 (228 bp; j). RNA was treated with (+) or without (−) reverse transcriptase (RT). Positive controls obtained from brain (B) or kidney (K). A representative gel for at least three independent experiments is shown. M, molecular mass