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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2018 Nov 16;661:107–116. doi: 10.1016/j.abb.2018.11.015

Table 3.

Kinetic Constants for the Amine Substrates for Bm-iAANAT3a

Amine Km
(μM)		 kcat
(s−1)		 (kcat/km)
(M−1 s−1)		 Relative
(kcat/km)
Tryptamine 97 ± 8.8 60 ± 1.2 (6.2 ± 0.6) × 105 33
Tyramine 63 ± 6.7 27 ± 0.6 (4.3 ± 0.5) × 105 23
Dopamine 330 ± 23 55 ± 1.1 (1.6 ± 0.1) × 105 8.4
Octopamine 18 ± 0.8 2.2 ± 0.02 (1.2 ± 0.05) × 105 6.3
Serotonin 1100±63 59 ± 1.0 (5.2 ± 0.3) × 104 2.7
Norepinephrine 140 ± 8.6 5.3 ± 0.07 (3.7 ± 0.2) × 104 1.9
Histamine 1700±98 33 ± 0.68 (1.9 ± 0.1) × 104 1.0
a

Kinetic constants for the amine substrates measured at one, fixed initial concentration of acetyl-CoA. Reactions were run at 22°C in 300 mM Tris pH 8.0, 150 μM DTNB, 500 μM acetyl-CoA, and the amine concentration was varied in the range 0.3 to 3.0 × Km.