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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Dev Biol. 2009 Jul 8;333(2):397–408. doi: 10.1016/j.ydbio.2009.06.044

Fig. 2.

Fig. 2.

In vitro DNA binding activity of ELT-1. (A) Binding to the sperm consensus site or sequence variants. Electrophoretic mobility shift assay of in vitro-generated ELT-1 protein with 32P-labeled oligonucleotide probes. See text for details. (B) Competitive binding assay. All samples contain equal amounts of ELT-1 protein plus 32P-labeled probe containing the sperm consensus site. Unlabeled probes were added at the indicated fold excess relative to the 32P-labeled probe to assess their relative binding affinities. See text for details.