Table 1.

Summary of fitted parameters. Synthetic data were generated as described in the text and then fit to derive estimates of steady state kinetic parameters using different equations and different means of data fitting. Here we list the methods for fitting and the chosen fitted parameters for each method. The values for k_cat, K_m and k_cat/K_m are shown. Values in brackets were calculated from the other parameters. For example, in the first row, k_cat/K_m was calculated from k_cat and K_m, whereas in the second row, K_m was calculated from estimates of k_cat and k_cat/K_m. Standard error estimates for calculated parameters were obtained by propagation of errors as described in the text. Note that the fitted parameters need not reproduce the input parameters used to generate synthetic because of the added errors and the limited set of data. A more complete data set with lower errors would return the input parameters.

method	fitted parameters	kcat (s−1)	Km (μM)	kcat/Km (μM−1s−1)
equations	kcat and Km	58.6 ± 25	44.4 ± 31	[1.32 ± 1.08]
	kcat and kSP	58.7 ± 25	[44.4 ± 23]	1.32 ± 0.37
simulation	kcat and Km	57.2 ± 5.9	27.2 ± 5.1	[2.11 ± 0.45]
	kcat and kSP	57.3 ± 6.1	[27.8 ± 4.1]	1.62 ± 0.21
simulation full reaction	kcat and Km	54.3 ± 4.9	23.6 ± 4.0	[2.30 ± 0.44]
	kcat and kSP	55.2 ± 3.9	[24.9 ± 2.2]	2.22 ± 0.12
input values		50	20	2.5