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Schematics of an “average” atom in the mesophilic (m) EcDHFR and in the psychropiezophilic (p) MpDHFR. The force constant ki, free energy difference between wells ΔGi, and transition barrier ΔG‡i, i = m or p, are indicated for the mesophile at standard T, P. Left well corresponds to the more favored conformation and right well corresponds to a more open conformation with lower probability.
