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. Author manuscript; available in PMC: 2019 Dec 7.
Published in final edited form as: Lab Chip. 2018 Oct 25;18(23):3617–3630. doi: 10.1039/c8lc00672e

Fig. 2. miRNA sequencing of brain-derived EVs from injured and control mice.

Fig. 2.

A. Brain-derived EV miRNAs were isolated using GluR2+ labeling and TENPO isolation and sequenced from pooled blast injured (415kPa) and control mice. DESeq normalized expression levels of individual miRNAs were plotted using a heat map. B. The five most abundantly expressed miRNAs from control and injured groups are plotted. C. Top 10 upregulated and downregulated miRNAs after the injury. D. 7 miRNAs biomarkers were selected and validated using qPCR. We used RNU6 for normalization. Expression levels from qPCR were compared to RNA sequencing. (R2 = 0.83 without and R2 = 0.62 with an outlier) Error bar indicates standard deviation of three technical replicates.