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. 2019 Jan 16;14(1):e0210553. doi: 10.1371/journal.pone.0210553

Fig 5. The binding specificities of anti-FP and anti-EP correlate with virus neutralization efficacy.

Fig 5

(A) The binding specificities of anti-FP and anti-EP to the target virus B4(E59) were examined by adding virus antigens from B4(E59) or C4(E36) as competitors in a competition ELISA. The binding efficiencies of anti-FP (B) and anti-EP (C) to FP or EP from the B4(E59) virus were evaluated by competitive ELISA assay. The antisera, competitors, and binding targets are indicated. Inhibition of viral neutralization by anti-FP or anti-EP against the B4(E59) (D) or C4(E36) (E) virus was examined by FP- or EP-adsorption experiments. BSA was used as the non-EV71 competitor/antigen, as a negative control in the competitive ELISAs and viral neutralization inhibition assays. The substantial neutralizing titers (≥1:128) conferred by BSA-pre-adsorbed anti-FP or anti-EP against the viruses were set as 100% normalized neutralization. The increase in the neutralization inhibition percentage was measured by pretreating anti-FP and anti-EP with FP or EP adsorption. Significant differences between groups are indicated by the following symbols: *, p<0.05; **, p<0.01; and ***, p<0.001. ns: no significant difference was present between the groups.