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. 2019 Jan 16;14(1):e0210553. doi: 10.1371/journal.pone.0210553

Fig 6. A VP1-specific antibody raised from FPs exhibits broad neutralization efficacy against EV71 subgenotype viruses.

Fig 6

(A) The purity of maltose-binding protein (MBP) fusions with the VP0, VP1, VP2, and VP3 EV71 capsid proteins was confirmed by SDS-PAGE analysis with Coomassie blue staining. (B) The neutralization activities of protein-preabsorbed antisera or antibodies against the B4(E59) or C4(E36) virus were measured. The tested antisera are labeled. MAB979 is a monoclonal VP2-specific antibody that neutralizes the B4(E59) virus and was used as a positive control for MBP-VP2 adsorption. The anti-VP1/C4 monoclonal antibody confers neutralization activity against C4(E36) and was used as a positive control for MBP-VP1 adsorption. (C) Measurement of EV71 subgenotype neutralization inhibition by preabsorbed anti-FP. The neutralizing titer provided by anti-FP pre-adsorbed with MBP against individual viruses was set as 100% normalized neutralization. The increase in the percentage of neutralization inhibition conferred by anti-FP pre-adsorbed with MBP-VP1 or MBP-VP2 was calculated and normalized to the MBP-adsorption groups. The EV71 virus strains used for this experiment are labeled. Significant differences between groups are indicated by the following symbols: *, p<0.05; **, p<0.01; and ***, p<0.001. ns: no significant difference was present between the groups.