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. 2019 Jan 16;14(1):e0209390. doi: 10.1371/journal.pone.0209390

Fig 4. Treatment of S. aureus biofilm with RIF and phage.

Fig 4

A. Combined action of phage and RIF on S. aureus biofilms. The legend is the same as for Fig 2. 20 wells were used for antibiotic-only controls, and simultaneous and sequential treatments, at each antibiotic concentration. The 2× and 10×MIC RIF-only control data are highlighted in yellow: some wells became turbid over the 48h of incubation (bacterial density ~ 1e9; data is the same as the points at the top of Fig 4B), and some wells remained clear (bacterial density ~ 1e5–1e7; data is the same as the points at the bottom of Fig 4B). The number of wells in each category is indicated below the corresponding box. B. Resistance status of phage-free controls. Bacterial densities are shown for each well, in solid orange dots for resistant wells and open green triangles for sensitive wells. Note that some of the cultures dosed with 2×MIC of RIF reached high densities (>1e8 cfu/mL) even though they appear to be sensitive to RIF when tested on plates containing 10×MIC RIF.