Table 3. Comparison of the experimental setup and parameters in Funck et al. [5] and our study.
Parameter | Funck et al. 2008 | This study |
---|---|---|
δOAT expression | Knockout | Gene induced under light |
Gowth conditions | MS medium, 110 μE, 9 h light, 22°C | Soil, 150 μE, 8 h light/ 16 h dark, 22°C/20°C; MS medium, 150 μE, 8 h light/ 16 h dark, 22°C/20°C |
CO2 supply | Ambient | Low (100 ppm) |
N-supply | 5 mM arginine + 0.5 mM glutamine; 10 mM ornithine + 0.5 mM glutamine |
High due to increased photorespiration |
Urea cycle intermediates | Accumulation in oat mutants under the N-supply given above; arginine and ornithine cannot be used as a sole nitrogen source in the absence of glutamine; urea can be metabolized without any difficulty | Accumulation under low CO2 conditions; alternative sink of nitrogen produced by photorespiration? |
Proposed/possible function of δOAT | Essential exit rout of nitrogen from urea cycle under physiological conditions | Possible function in glutamate homeostasis and/or production of glutamate for photorespiration |