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. 2019 Jan 16;14(1):e0210342. doi: 10.1371/journal.pone.0210342

Table 3. Comparison of the experimental setup and parameters in Funck et al. [5] and our study.

Parameter Funck et al. 2008 This study
δOAT expression Knockout Gene induced under light
Gowth conditions MS medium, 110 μE, 9 h light, 22°C Soil, 150 μE, 8 h light/ 16 h dark, 22°C/20°C;
MS medium, 150 μE, 8 h light/ 16 h dark, 22°C/20°C
CO2 supply Ambient Low (100 ppm)
N-supply 5 mM arginine + 0.5 mM glutamine;
10 mM ornithine + 0.5 mM glutamine
High due to increased photorespiration
Urea cycle intermediates Accumulation in oat mutants under the N-supply given above; arginine and ornithine cannot be used as a sole nitrogen source in the absence of glutamine; urea can be metabolized without any difficulty Accumulation under low CO2 conditions; alternative sink of nitrogen produced by photorespiration?
Proposed/possible function of δOAT Essential exit rout of nitrogen from urea cycle under physiological conditions Possible function in glutamate homeostasis and/or production of glutamate for photorespiration