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. 2019 Jan 4;17(1):e2006926. doi: 10.1371/journal.pbio.2006926

Fig 3. Differential utilization of upstream autophagy components by RNA viruses.

Fig 3

(A and B) Cells were treated with rapamycin or infected with PV (6 hpi), DENV (24 hpi), or ZIKV (24 hpi). Cell lysates were harvested and immunoblotted with phospho-S6K or phospho-AMPK antibodies. (C) Cells were treated with 0.5, 5, or 50 μM of MBL56 and infected with PV at an MOI of 0.1 PFU/cell for 6 hours. (D) WT cells were pretreated with 1, 5, or 10 μM of SAR405 and infected with DENV or ZIKV at an MOI of 0.1 PFU/cell for 24 hours (DENV) or 48 hours (ZIKV). WT cells were pretreated with 10 μM of SAR405 and infected with PV at an MOI of 0.1 PFU/cell for 6 hours. All data are represented as mean +/− SEM. *Indicates significant P value of <0.05, **P value < 0.01, ***P value < 0.001, ****P value > 0.0001 by an unpaired t test. See also S3 Data. AMPK, AMP-activated protein kinase; DENV, dengue virus; hpi, hours post infection; MOI, multiplicity of infection; PFU, plaque-forming units; PV, poliovirus; WT, wild-type; ZIKV, Zika virus.