Fig. 4.

Distal nephron-specific Per1 KO mice have increased Na transporter expression. A: generation of distal nephron-specific Per1 KO mice. Floxed Per1 schematic shows placement of loxP sites flanking exons 2–8 and location of the neomycin cassette, which all mice still contained. Solid arrows denote genotyping primers to detect deletion of exons 2–8. Ksp-cadherin genotyping was performed according to protocol in Ref. 17. B: PCR was performed on inner medulla genomic DNA of control and distal nephron-Per1 KO mice to confirm recombination in distal nephron Per1 KO mice. C: relative gene expression of α-epithelial Na channel (αENaC) and the sodium chloride cotransporter (NCC) in the cortex of fl/fl control (black) and distal nephron-specific Per1 KO (gray) mice on a normal diet. Significance was determined by Studentʼs t-test. *P < 0.05 vs. fl/fl control; n = 3 male mice per group.