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. 2017 Nov 22;314(3):C349–C365. doi: 10.1152/ajpcell.00218.2017

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Fig. 7. — Effect of depleting RAB11A or RAB27B on the distribution, size, intensity, and number of DFVs. Bladders were transduced with adenoviruses encoding scrambled (Scr)-shRNA, RAB11A-shRNA-1, or RAB27B-shRNA-a. A: confocal images showing the distribution of RAB27B and RAB11A in transduced urothelial tissue. The nonmucosal surfaces of the urothelium were labeled using an antibody to CLD4. B: summary statistics for the size, intensity, and number of RAB27B-positive vesicles in cells transduced with the indicated shRNA. None of the values are significantly different from scrambled (Scr)-shRNA controls (assessed using t-tests). C: confocal images showing the distribution of RAB27B and RAB11A in transduced urothelial tissues. The cortical actin cytoskeleton of the urothelium was labeled using rhodamine phalloidin. D: summary statistics for the size, intensity, and number of RAB11A-positive vesicles in cells transduced with the indicated shRNA. None of the values are significantly different from scrambled (Scr)-shRNA controls (assessed using t-tests).