Figure 4.

Changes in subventricular zone (SVZ) cells after LPC-induced demyelination. (A,B) Immunolabeling for YFP, Olig2, and DAPI showing increased thickness of the dorsal SVZ 28 days after LPC injection (B) compared with PBS-injected control (A). Very few Olig2+ cells are found in the control SVZ after PBS injection, whereas several Olig2+ cells are detected in the thickened SVZ after LPC injection. (C,D) Immunolabeling for doublecortin (Dcx) and PSA-NCAM showing that many of the YFP+ cells co-express Dcx and PSA-NCAM. (D) represents a higher magnification of the boxed area in C. (E–H) Immunolabeling for YFP, Dcx, and (PSA-NCAM) showing that most of the Dcx/PSA-NCAM+ cells are confined to the SVZ in control (E), whereas LPC-injected animals show a greater number of Dcx+ PSA-NCAM+ cells migrating dorsally into the SVZ (F–H). (H) is a higher magnification image of the boxed area in (F). (I,J) Immunolabeling for YFP, Olig2, and PSA-NCAM near the lesion (dotted line) at 14 dpl. There is a dense cluster of PSA-NCAM+ cells in the lateral angle of the SVZ (right side in I). Higher magnification of the boxed area in (I), showing a YFP+ cell that is also PSA-NCAM+ (J, white arrow). There is a cluster of strongly Olig2+ cells inside the lesion (arrowheads in (J)), and weakly Olig2+ PSA-NCAM+ cells are detected at the lesion border (pink arrows in (J)). (K,L) PSA-NCAM+ cells, some of which are YFP+, appear to be migrating in the corpus callosum in clusters (K), and many of them have a tadpole-shaped unipolar morphology (L). Scale bar: 50 μm.