Table 1.
Primary antibodies used.
| Antibody | Host Species | Source | Dilution |
|---|---|---|---|
| Dcx | Rabbit | Cell Signaling Technology (Danvers, MA, USA) | 1:300 |
| NG2 | Rabbit | EMD Millipore (Burlington, MA, USA) | 1:500 |
| Pdgfra | Goat | R&D Systems (Minneapolis, MN, USA) | 1:1000 |
| CC1 (Quaking 7) | Mouse | EMD Millipore (Burlington, MA, USA) | 1:100 |
| MBP, smi99 antibody | Mouse | Covance (Princeton, NJ, USA) | 1:3000 |
| Smi32 | Mouse | Covance (Princeton, NJ, USA) | 1:1000 |
| GFP | Chick | Aves Labs (Tigard, OR, USA) | 1:1000 |
| Olig2 | Mouse | EMD Millipore (Burlington, MA, USA) | 1:1000 |
| Olig2 | Rabbit | Novus Biologicals (Littleton, CO, USA) | 1:1000 |
| PSA-NCAM, 12E3 antibody | Mouse | Dr. Tatsunori Seki (Tokyo Medical University, Tokyo, Japan) | 1:1000 |
| GFAP | Rabbit | DAKO-Agilent (Santa Clara, CA, USA) | 1:2000 |
Dcx: Doublecortin; Pdgfra: Platelet-derived growth factor receptor α; MBP: Myelin basic protein; Smi32: Non-phosphorylated neurofilaments; GFP: Green fluorescent protein; Olig2: Oligodendrocyte transcription factor 2; PSA-NCAM: Polysialic acid-neural cell adhesion molecule; GFAP: Glial fibrillary acidic protein.