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. 2019 Jan 16;8:e40806. doi: 10.7554/eLife.40806

Key resources table.

Reagent type (species) or
resource		 Designation Source or reference Identifiers Additional information
Genetic reagent
(Drosophila
melanogaster)		 ova[1] This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 ova[4] This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 c587-GAL4 (Song et al., 2004)
(DOI: 10.1242/dev.01026)		 RRID:BDSC_67747
Genetic reagent
(Drosophila
melanogaster)		 Dad-lacZ (Tsuneizumi et al., 1997)
(DOI: 10.1038/39362)		 RRID:DGGR_118114
Genetic reagent
(Drosophila
melanogaster)		 bam-GFP (Chen and McKearin, 2003) RRID:DGGR_118177
Genetic reagent
(Drosophila
melanogaster)		 piwi[2] (Lin and Spradling, 1997) RRID:BDSC_43319
Genetic reagent
(Drosophila
melanogaster)		 piwi[3] (Lin and Spradling, 1997) RRID:BDSC_12225
Genetic reagent
(Drosophila
melanogaster)		 GFP-piwi Katalin Toth
(California Institute of
Tchnology)
Genetic reagent
(Drosophila
melanogaster)		 118E-10 Lori Wallrath
(University of Iowa)
Genetic reagent
(Drosophila
melanogaster)		 118E-15 Lori Wallrath
(University of Iowa)
Genetic reagent
(Drosophila
melanogaster)		 6 M-193 Lori Wallrath
(University of Iowa)
Genetic reagent
(Drosophila
melanogaster)		 39C.72 Lori Wallrath
(University of Iowa)
Genetic reagent
(Drosophila
melanogaster)		 dLsd1-GFP Yu Yang (Institute of
Biophysics, Chinese
Academy of Science)
Genetic reagent
(Drosophila
melanogaster)		 EGFP-RNAi Bloomington Drosophila
Stock Center		 (#41553)
Genetic reagent
(Drosophila
melanogaster)		 RFP-HP1a Bloomington Drosophila
Stock Center		 (#30562)
Genetic reagent
(Drosophila
melanogaster)		 UAS-Dcr2; nos-GAL4 Bloomington Drosophila
Stock Center		 (#25751)
Genetic reagent
(Drosophila
melanogaster)		 tub-GAL4 Bloomington Drosophila
Stock Center		 (#5138)
Genetic reagent
(Drosophila
melanogaster)		 tub-GAL80ts Bloomington Drosophila
Stock Center		 (#7016, #7018)
Genetic reagent
(Drosophila
melanogaster)		 Df(2L)BSC144 Bloomington Drosophila
Stock Center		 (#9504)
Genetic reagent
(Drosophila
melanogaster)		 attP2 Bloomington Drosophila
Stock Center		 (#25710)
Genetic reagent
(Drosophila
melanogaster)		 In(1)wm4h Kyoto Stock Center (#101652)
Genetic reagent
(Drosophila
melanogaster)		 Df(2L)ED737 Kyoto Stock Center (#150520)
Genetic reagent
(Drosophila
melanogaster)		 ova-RNAi Vienna Drosophila
Research Center		 (#102156)
Genetic reagent
(Drosophila
melanogaster)		 piwi-RNAi Vienna Drosophila
Research Center		 (#101658)
Genetic reagent
(Drosophila
melanogaster)		 panx-RNAi Vienna Drosophila
Research Center		 (#102702)
Genetic reagent
(Drosophila
melanogaster)		 EGFP-5xBoxB Vienna Drosophila
Research Center		 (#313408)
Genetic reagent
(Drosophila
melanogaster)		 lacO-GFP-Piwi Vienna Drosophila
Research Center		 (#313394)
Genetic reagent
(Drosophila
melanogaster)		 lacI-HP1a; lacO-
GFP-Piwi		 Vienna Drosophila
Research Center		 (#313409)
Genetic reagent
(Drosophila
melanogaster)		 nos-Cas9 Jianquan Ni
(Tsinghua University)
Genetic reagent
(Drosophila
melanogaster)		 pCasper4-ova-g This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 GFP-ova This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-ova This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-piwi This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-ova1-249 This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-ova250-486 This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-ova388-623 This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-ova1-388 This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-HP1a::dLsd1 This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-λN-ova This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 UASP-lacI-ova This paper See Materials and
methods
Genetic reagent
(Drosophila
melanogaster)		 lacO-terminator-GFP-Piwi This paper See Materials and
methods
Recombinant DNA
reagent		 UASP-λN Julius Brennecke
(Institute of Molecular
Biotechnology)
Recombinant DNA
reagent		 UASP-lacI
Recombinant DNA
reagent		 lacO-GFP-Piwi
Recombinant DNA
reagent		 pGBKT7 Clontech (Cat#630443)
Recombinant DNA
reagent		 pGAD Clontech (Cat#630442)
Antibody rabbit polyclonal
anti-pMad		 Ed Laufer (Columbia
Universtity Medical
Center)		 RRID:AB_2617125 IHC(1:1000)
Antibody rabbit polyclonal
anti-β-galactosidase		 MP Biologicals
(Cat#0855976)		 RRID:AB_2687418 IHC(1:3000)
Antibody mouse monoclonal
anti-α-Spectrin		 Developmental Studies
Hybridoma Bank		 IHC(1:50)
Antibody mouse monoclonal
anti-Tubulin		 Developmental Studies
Hybridoma Bank		 RRID:AB_1157911 WB(1:2000)
Antibody rabbit polyclonal
anti-mCherry		 BioVision (cat#5993) RRID:AB_1975001 WB (1:2000)
Antibody rabbit polyclonal
anti-GFP		 Life (cat#A11122) RRID:AB_221569 IHC(1:1000)
WB(1:10000)
Antibody polyclonal
anti-rabbit IgG-HRP		 ZSJQ-BIO (cat#ZB2301) WB(1:10000)
Antibody rabbit polyclonal
anti-H3K4me2		 Abcam (cat#ab7766) RRID:AB_732924
Antibody mouse monoclonal
anti-RNA polymerase II		 Abcam (cat#ab817) RRID:AB_306327
Antibody mouse monoclonal
anti-Flag		 Sigma (cat#F1804) RRID:AB_439685 IHC(1:300)
WB(1:6000)
Chemical
compound, drug		 4’,6’-diamidino-2-
phenylindole		 Sigma
(cat#10236276001)
Commercial
assay or kit		 anti-Flag resin Sigma (cat#A2220) RRID:AB_10063035
Commercial
assay or kit		 GFP-Trap agarose Chromoteck
(cat#gta-10)
Commercial
assay or kit		 RFP-Trap agarose Chromoteck
(cat#rta-10)
Commercial
assay or kit		 Qiagen Plasmid
Midi Kit		 Qiagen (#12145)
Commercial
assay or kit		 Immobilon Western
Chemiluminescent
HRP Substrate Kit		 Millipore
(cat#WBKLS0500)
Commercial
assay or kit		 HiScript II Q RT
SuperMix		 Vazyme Biotech
(cat#R223-01)
Commercial
assay or kit		 ChamQ SYBR qPCR
master Mix		 Vazyme Biotech
(cat#Q331)
Commercial
assay or kit		 Oligo d(T)25 Magnetic
beads		 NEB (cat#S1419S)
Commercial
assay or kit		 NEBNext Ultra IIDNA
Library Prep Kits for
Illumina		 NEB (cat# E7645S)
Commercial
assay or kit		 VAHTS Small RNA
Library Prep Kit
for Illumina		 Vazyme Biotech
(cat#NR801)
Commercial
assay or kit		 VAHTS Universal DNA
Library Prep Kit		 Vazyme Biotech
(cat#ND607)
Commercial
assay or kit		 TruePrep Index Kit Vazyme Biotech
(cat#TD202)
Software,
algorithm		 GraphPad Prism GraphPad Prism
(https://graphpad.com)		 RRID:SCR_002798
Sequenced-based
reagent		 RT-qPCR primers This paper See Supplementary file 1,
Table 2