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. 2018 Dec 10;7:e42908. doi: 10.7554/eLife.42908

Table 1. Quantitative-BRET measurements of Gβγ release from different Gα constructs.

Averaged Nano-Luc intensity, basal BRET ratio, and ΔBRET ratio were summarized (N = 3–4, ±SD).

NLuc intensity  Basal BRET ratio ΔBRET ratio
M2R-Gαi1(αa-αb) (13.85 ± 0.36) x105 0.301 ± 0.003 0.031 ± 0.002
β2AR- Gαs(αa-αb) (11.91 ± 2.80) x105 0.310 ± 0.007 0.002 ± 0.006
M2R-Gαi1(αb-αc) (8.08 ± 1.77) x105 0.310 ± 0.014 0.029 ± 0.006
β2AR- Gαs(αb-αc) (8.64 ± 2.62) x105 0.311 ± 0.014 0.005 ± 0.005
D2R-Gαi1(αa-αb) (23.29 ± 1.46) x105 0.312 ± 0.005 0.028 ± 0.006
β1AR- Gαs(αa-αb) (24.51 ± 1.09) x105 0.315 ± 0.007 0.007 ± 0.003
D2R-Gαi1(αb-αc) (5.58 ± 4.31) x105 0.287 ± 0.001 0.032 ± 0.004
β1AR- Gαs(αb-αc) (7.77 ± 0.34) x105 0.279 ± 0.004 0.008 ± 0.003