Table 4.

NLR coverage in commercial potato variety Innovator following RenSeq and whole‐genome sequencing

	Innovator
	RenSeq	Whole‐genome sequencing
Total gigabase pairs	0.778	69.04	28.99	19.33	9.3	0.777
Gene name	RenSeq_all	sub88 [all]	sub36	sub24	sub12	sub01
Rpi‐R1 ΔT4109	100.00	100.00	100.00	99.34	89.76	7.36
Rpi‐R2‐like	100.00	100.00	100.00	95.13	93.12	20.48
Rpi‐R3a	100.00	100.00	98.29	97.04	91.22	11.33
Rpi‐R3b	97.72	99.22	97.30	96.96	71.63	0.00
Rpi‐R3b G1696/G3111	100.00	100.00	100.00	100.00	83.15	0.00

DRenSeq was conducted on potato variety Innovator and compared to whole‐genome sequencing (WGS). For the comparison between RenSeq and WGS, subsamples of WGS reads were obtained. The sequence volume of WGS reads compared to RenSeq reads are shown in gigabases and x sequence volume [sub 01 =  equal amount to RenSeq; sub12 = 12× WGS compared to RenSeq; sub24 = 24× WGS compared to RenSeq; sub36 = 36× WGS compared to RenSeq; sub88 = 88× WGS compared to RenSeq (in this case all WGS data). The IDs of the Resistance to Phytophthora infestans (Rpi) nucleotide‐binding, leucine‐rich‐repeat resistances are shown. The representation of individual, full‐length Rpi genes was calculated by extracting the sequence coverage of dRenSeq‐mapped reads to the reference coding DNA sequence (CDS). WGS reads were mapped under the same stringent mapping condition used for dRenSeq. Highlighted in green are Rpi genes that achieved 100% representation and are therefore classified as ‘present’.