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. 2018 Oct 24;17(2):451–460. doi: 10.1111/pbi.12989

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© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The genetic transformation process and results of transient transformation. (a) Simultaneous selection and regeneration on WPM‐reg plates. (b) Multiple green transgenic calli. (c) Transformed shoots regenerated from calli on WPM‐elo media. (d) A regenerated transgenic whole plant on rooting medium. (e) A transgenic plant under greenhouse conditions. (f–i) Transient GFP fusion protein expression assay in nucleus. (f) bright field; (g) DAPI; (h) green fluorescence; (i) overlay. Scale bar = 20 μm.