Figure 2.

CBSV and UCBSV VPg's interact with cassava nCBP‐1 and nCBP‐2. (a) Immunoprecipitation of YFP‐cassava eIF4E isoform fusions, expressed in N. benthamiana, co‐immunoprecipitate purified CBSV‐Nal VPg‐3xFLAG that was added to plant extracts. (b) Yeast two‐hybrid constructs consist of B42 activation domain (AD) fused to the CBSV Naliendele VPg and UCBSV T04 VPg, and LexA DNA‐binding domain (BD) bound to cassava, Me, eIF4E family members. Blue coloration represents β‐galactosidase activity from activation of lacZ reporter gene by protein–protein interaction. Five yeast transformants are displayed on the dropout medium SD Gal/Raf SD‐UTH. Positive control is shown in the dashed red box (TuMV VPg‐AD and A. thaliana, At, eIF(iso)4E‐BD).