Figure 3.

Cas9 induces INDELs at nCBP‐1 and nCBP‐2 gRNA target sites in BS05 transgenic lines. (a) Sequences at the junction of the first exon–intron boundary were selected for targeting of the Cas9 nuclease. Lengths of nCBP‐1 and nCBP‐2 genes are to nucleotide scale (top bar). Exons are denoted by solid blocks and introns are represented as dashed lines. Arrowheads indicate the 3′ terminus. (b) Diagram of the protospacer adjacent motif (PAM) and guide RNA (gRNA) targeting nCBP‐2. (c) Diagram of the PAM and gRNA targeting nCBP‐1. (d) INDELs of nCBP‐1 and nCBP‐2 in mutant lines ncbp‐1 #1, ncbp‐2 #6, ncbp‐1 ncbp‐2 #2, and ncbp‐1 ncbp‐2 #8. Upper and lower cases denote exonic and intronic sequence, respectively. Red boxes indicate INDELs.