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. 2018 Oct 5;17(2):421–434. doi: 10.1111/pbi.12987

Figure 7.

Figure 7

ncbp‐1 ncbp‐2 double mutant storage roots are less symptomatic and accumulate less virus. (a), storage root sections were assessed on a 1–5 scale, one corresponding with asymptomatic and five corresponding with necrosis spanning the diameter of root. ncbp‐1 ncbp‐2 storage roots are significantly less symptomatic than wild‐type at 12–14 weeks post bud‐graft inoculation with CBSV Naliendele isolate. Points represent average scores of all storage root sections from a single plant. Data from three experimental replicates were pooled. Whiskers span the interquartile range, solid bars indicate the median of scores, + indicates the mean of scores. Statistical significance was detected by Welch's t‐test, n ≥ 19, α = 0.05, *≤0.05, **≤0.01. (b), quantitative real‐time PCR analysis reveals that ncbp‐1/2 storage roots accumulate less virus than wild‐type. CBSV HAM1‐LIKE levels are reported as normalized relative quantities (NRQs) relative to PP2A4. Data from three experimental replicates were pooled. Significant differences were detected with a Mann–Whitney U‐test, n ≥ 19, α = 0.05.