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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Biomed Microdevices. 2017 Nov 11;19(4):101. doi: 10.1007/s10544-017-0244-6

Figure 1.

Figure 1

(A) Schematic of the device to carry out the continuous-flow ELISA. Cell perfusate is carried the first mixing channel where it conjugates with the biotinylated beads. The antigen-tagged beads and the secondary antibody conjugate in the second mixing channel. The fluorescently labeled beads are detected under a fluorescence microscope at the outlet, (B) Schematic of the analytical model, and (C) Plot representing the relationship between the channel dimensions, flow rate, and the concentration of the antibody.