Table 1.

Human AMH mRNA contains let-7 binding sites at its 3′UTR. Let-7 binding sites in the AMH gene were predicted using the web-based program RNAhybrid. Preliminary bioinformatic analysis revealed several putative let-7 binding sites in the AMH mRNA. In particular, a binding site was predicted at position 775 in the 3′UTR of human AMH mRNA. Partial sequences of AMH (in red) and let-7 are shown.

miRNA	Position	Sequence	Binding energy
Let-7b	775	5′G A UGGACACCA U3′ CACACGGCC GC UGCCUU GUGUGUUGG UG AUGGAG 3′UUG A U5′	−24.9 kcal/mol
Let-7c	769	5′C GCAC A UGGACACCA U3′ AGCCA ACGGCCGC UGCCUU UUGGU UGUUGGUG AUGGAG 3′ A A U5′	−22.8 kcal/mol
Let-7i	222	5′G UAU G C3′ AACAGC GAUAUGCCUU UUGUCG UUAUAUGGAG 3′ UGU G G U5′	−20.6 kcal/mol
Let-7j	772	5′C CGGCC GACACC U3′ CAGCACA AGCUG AUGCCUU GUCGUGU UUGAU UAUGGAG 3′UAUA U5′	−24.3 kcal/mol
Let-7j	222	5′G U U C3′ AACAGC AUGAG AUGCCUU UUGUCG UGUUU UAUGGAG 3′ UA GAU U5′	−21.7 kcal/mol
Let-7k	775	5′G AGCUGGACA A U3′ CACACGGCC CCUGCCUU GUGUGUUGG GGAUGGAG 3′ A U5′	−23.7 kcal/mol