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. 2018 Aug 8;100(1):139–148. doi: 10.1093/biolre/ioy179

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Figure 7. — Castration minimizes Ang II-induced cardiac fibrosis, which is restored by testosterone. Intact, castrated and castrated with testosterone-replaced rats were infused with vehicle or Ang II for 4 weeks as described in the Methods section. After the end of Ang II infusion, heart was removed and processed for immunohistochemistry. Masson trichrome staining revealed intracardiac collagen deposition (intense blue staining). Photomicrographs are representative images of at least three animals from each experimental group. Presented below is the percentage of total fibrosis area as calculated as the summed blue-stained areas divided by total ventricular area. *P < 0.05 compared with respective vehicle-infused group. ^§P < 0.05 compared with intact Ang II-infused group. CAS—castrated, CAS+T—castrated with testosterone replacement.