FIGURE 2.

Motility of B. cenocepacia H111 and PDE/DGC mutants in semi-solid medium. (A) Swimming motility. Overnight cultures of B. cenocepacia H111 and mutant derivatives were adjusted to OD₆₀₀ 4 and inoculated into swim plates. Plates were incubated at 37°C and the relative swimming diameter was measured after 10 h. Strains with disruption of genes encoding GGDEF-only proteins are highlighted in light gray, composite GGDEF-EAL protein mutants in gray, EAL-only and HD-GYP-domain protein mutants in dark gray, respectively. Data was normalized to wild type results and shows mean swimming diameter and standard derivations of three individual experiments. Asterisks indicate P < 0.05 with unpaired t-test. (B) Swarming motility. Overnight cultures of B. cenocepacia H111 and mutant derivatives were adjusted to OD₆₀₀ 4.0 and inoculated on top of a swarm plate. Plates were incubated at 37°C and pictures were captured after 24 h. Data shown here are representatives of three independent experiments.