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. 2019 Jan 10;9:3286. doi: 10.3389/fmicb.2018.03286

FIGURE 5.

FIGURE 5

Biofilm formation in microtiter plates by the B. cenocepacia rpfR mutant under various medium conditions. Cells were grown in ABnoNaCl supplemented with carbon sources as indicated (see material and methods for concentrations) under static conditions at 37°C for 24 h. Subsequently, the amount of biofilm on the wells and on the pegs, respectively, was determined via a CV staining assay. Graphs show OD590nm values of CV bound by the ΔrpfR mutant normalized to values obtained from the wild type. Error bars indicate standard deviations of 4 replicates per strain, and data shown here are representatives of three independent experiments.