Table 2.
Plasmids used in this study.
| Plasmid | Characteristics | Reference |
|---|---|---|
| pEX18Gm | Suicide vector for cdpA mutant construction | Hoang et al., 1998 |
| Pmini-Tn7-kan-gfp | Delivery vector for mini-Tn7-kan-gfp, Kmr | Norris et al., 2010 |
| pRK600 | Helper plasmid in tri- and four-parental matings; ori-ColE1 RK-mob+ RK-tra+; Cmr | Kessler et al., 1992 |
| pUX-BF13 | Helper plasmid providing the Tn7 transposition functions in trans; mob+ ori-R6K; Ampr | Bao et al., 1991 |
| pDAI-SceI-pheS | Cloning vector containing the I-SceI endonuclease and pheS; Tetr | Fazli et al., 2015 |
| pDONRPEX18Tp-SceI-pheS | Gateway compatible gene replacement vector based on SceI and pheS; Tpr | Fazli et al., 2015 |
| pDONRPEX18Gm-SceI-pheS | Gateway compatible gene replacement vector based on SceI and pheS; Gmr | Fazli et al., 2015 |
| pENTRPEX18Tp-SceI-pheS-bcal0430 | Gene replacement vector containing the bcal0430 deletion allele, Tpr | This study |
| pENTRPEX18Gm-SceI-pheS-bcal1635 | Gene replacement vector containing the bcal1635 deletion allele, Gmr | |
| pENTRPEX18Tp-SceI-pheS-bcal1975 | Gene replacement vector containing the bcal1975 deletion allele, Tpr | |
| pENTRPEX18Gm-SceI-pheS-bcal2852 | Gene replacement vector containing the bcal2852 deletion allele, Gmr | |
| pENTRPEX18Tp-SceI-pheS-bcam0748 | Gene replacement vector containing the bcam0748 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam1161 | Gene replacement vector containing the bcam1161 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam1554 | Gene replacement vector containing the bcam1554 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam1670 | Gene replacement vector containing the bcam1670 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam2256 | Gene replacement vector containing the bcam2256 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam2822 | Gene replacement vector containing the bcam2822 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam2836 | Gene replacement vector containing the bcam2836 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcas0398 | Gene replacement vector containing the bcas0398 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcal0621 | Gene replacement vector containing the bcal0621 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcal2449 | Gene replacement vector containing the bcal2449 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam1160 | Gene replacement vector containing the bcam1160 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcal0652 | Gene replacement vector containing the bcal0652 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcal1100 | Gene replacement vector containing the bcal1100 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcal2749 | Gene replacement vector containing the bcal2749 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcal3188 | Gene replacement vector containing the bcal3188 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam0158 | Gene replacement vector containing the bcam0158 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcam2426 | Gene replacement vector containing the bcam2426 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcas0263 | Gene replacement vector containing the bcas0263 deletion allele, Tpr | |
| pENTRPEX18Tp-SceI-pheS-bcas0378 | Gene replacement vector containing the bcas0378 deletion allele, Tpr |