Figure 2.

FCs immunization attenuates initial atherosclerotic lesion. (A) Schematic illustration of the prevention experimental setup. (B) Evaluation of en face lesion size of the whole aorta was carried out by Oil Red O staining. (C) Plaque size in the three-valve area of the aortic root was assessed by hematoxylin and eosin staining. (D) Collagen content in the aortic root was determined by Masson's Trichrome staining as the percentage of the total lesion area. Scale bars, 200 μm. All data are presented as mean ± SEM and are representative of all mice. n = 5–6 in each group; ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.