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FCs immunization results in a specific antibody response in initial atherosclerosis. (A) ELISA was performed to measure the specific antibodies to FCs and macrophages as well as immunoglobulin isotypes including IgG1 and IgG2a titers. Data are shown at plasma dilutions of 1: 800 for each group (n = 8). OD, optical density. (B) Peritoneal macrophages and FCs were fixed and stained with PBS-immune, Macrophages-immune or FCs-immune plasma (dilution 1:50). Scale bars, 50 μm.
