Figure 7.

FCs immune plasma blocks the process of FCs formation. (A) For the FCs formation inhibition study, the macrophages were incubated in duplicate with or without plasma (50 μL/mL) from FC-immune ApoE^−/− mice for 3 h in the presence of DiI-oxLDL. (B) Further investigation of the effect of immune plasma on FCs formation. Macrophages were treated with or without plasma (50 μL/mL) from PBS-immune, macrophages-immune, or FC-immune ApoE^−/− mice for 1 h. After washed, cells were cultured with 30 μg/mL DiI-oxLDL for 3 h (B). Internalized oxLDL was revealed by DiI fluorescence, and six random fields per condition were captured by High Content Screening. A representative image is shown for each condition. Scale bars, 50 μm. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.