Figure 7.

NET is the main pathway to LTD in severe PTB (A) Evaluation of alpha-1-antitrypsin (α1AT) levels before and after 60 days of ATT inpatients with radiological improvement or not after 60 days of ATT. (B) Relation of α1AT levels and neutrophil counts. (C,D) Relation of α1AT levels with culture and AFB conversion, respectively. (E) Relation of citrullinated histone H3 (cit-H3) levels and neutrophil counts. (F) Comparison of cit-H3 levels before and after 60 days of ATT between patients who presented radiological improvement or not after 60 days of ATT. (G) Comparison of cit-H3 levels in patients who presented or not cavity formation. (H,I) Relation of α1AT levels with AFB and culture conversion, respectively. In graphs “B” and “E”, “Neutrophil High” refers to neutrophilia (count ≥ 7,500 cells/mm³) and “Neutrophil Low” refers to normal or low neutrophils count (count < 7,500 cells/mm³). α1AT and cit-H3 levels are presented as ng/mL. Data in each figure are expressed as mean ± SD. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.0001 by 1-way ANOVA followed by Newman-Keuls test. (J) Overlay of ROC curve at day 0 and 60 of ATT, revealed that only neutrophil, cit-H3 and α1AT day showing are related with LTD in PTB. The logistic regression model was built using STATA 14 and the strongest significant predictors variables were selected with a p < 0.05.