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. 2019 Jan 10;12:72. doi: 10.3389/fnsys.2018.00072

FIGURE 1.

FIGURE 1

(A) Timeline of experimental manipulations and histological assessment of dorsal CA3/DG cannulae placements from Experiments 1 and 2. (B) Representative images show DAPI-stained sections (converted to grayscale) with tracts of 22G stainless steel guide cannulae positioned above the dorsal CA3/DG at target coordinates, relative to Bregma: AP –4 mm, ML ±3mm, DV –2.6 mm from skull surface. While no visible damage was observed in most rats, 28G microinjectors protruded an additional 1 mm below cannulae tips, centering the infusion between DG upper and lower blades. (C) Representative image from Experiment 1 brain section processed with fluorescence in situ hybridization (FISH) to label Arc mRNA (Cy3; red channel), counter-stained with DAPI. Strong induction of the immediate-early gene Arc is evident in both upper and lower blades of DG. (D) Schematics show position of foci of infusions based on cannulae tracts in each rat from Experiment 1 (n = 10), Experiment 2 (n = 17), and separate study carried out to verify effects of vehicle vs. muscimol infusion on Arc mRNA expression as a read-out of neural activity (n = 6). Numerical values on brain sections indicate AP coordinate, relative to Bregma.