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. 2019 Jan 10;9:1984. doi: 10.3389/fpls.2018.01984

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Copyright © 2019 Barabasz, Palusińska, Papierniak, Kendziorek, Kozak, Williams and Antosiewicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Organ- and developmental-regulation expression of selected ZIP genes in N. tabacum (A–H). Analysis was performed by RT-qPCR. Plants were grown at control medium. Transcript levels were monitored in the whole roots and leaves of plants grown for 4 and 6 weeks, and after 9 weeks in the apical (A) and basal (B) segments of roots (R), in stems (S), in young upper leaves (UL), and old lower leaves (LL). The level of genes transcript was normalized to PP2A expression level. Values correspond to means ± SD (n = 3); those with the ratio greater than two are considered significantly different.