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. 2019 Jan 10;9:1984. doi: 10.3389/fpls.2018.01984

FIGURE 5.

FIGURE 5

Expression pattern of NtZIP4A and NtZIP4B in N. tabacum under various Zn conditions (A1,B1,A2,B2) and in the presence of Cd (C1,C2). Plants were grown in the quarter-strength Knop’s medium (control) and then transferred to the control medium supplemented with the following Zn or Cd concentrations: (i) 50 μM Zn for 1 day (Zn excess-1d); (ii) without Zn for 4 days (Zn deficit); (iii) plants grown at Zn-deficiency for 4 days were transferred to the control medium for 2 days (Zn replete); (iv) 50 μM Zn for 21 days (Zn excess-21d); (v) 4 μM Cd for 3 days (Cd). RT-qPCR analyses was performed on cDNA prepared from the two old lower leaves counting from the base (LL), two young upper leaves (UL), apical part of roots (RA), and basal part of roots (RB) of N. tabacum. Gene expression was normalized to the PP2A level. Values correspond to means ± SD (n = 3); those with the ratio greater than two are considered significantly different.