FIGURE 9.

Plasma membrane localization of the NtZIP4B-GFP fusion protein transiently expressed in the lower epidermis of tobacco leaf. Laser scanning confocal micrographs include: (A) GFP fluorescence concentrated to the cell’s contours, autofluorescence of the cell wall of non-transformed cells is below the detection limit for settings used for visualization of the GFP signal; (B) propidium iodide red fluorescence of the cell’s contours; (C) overlapped GFP and propidium iodide signal – both signals with the same localization; (D) bright field. (C,D) White arrows indicate localization of the tonoplast at the border between two vacuoles (v), and at the border between the vacuole and the cytoplasm (c) pushed into a protruding end of a cell. Green and red fluorescence is not present in the tonoplast (at the border between the vacuoles, and between the vacuole and the cytoplasm within the protruding end of a cell).