Fig. 5.

Detection of skin prion-seeding activity by RT-QuIC in Tg40h mice. Prion-seeding activity of thigh (a) back (b) and belly (c) skin homogenates from the Tg40h mice inoculated with sCJDMM1 brain homogenate and killed at 4, 8, 12, 16, 20, 22, or 24 wpi was detected by RT-QuIC assay with recombinant hamster PrP90-231 as the substrate. PBS represents-negative control Tg40h mice that were inoculated intracerebrally with PBS for 26 weeks. d Scatter plot of average of skin prion-seeding activity of thigh, back, or belly from sCJDMM1-inoculated Tg40h mice at 4,  12, 16, 20, 22, and 24 wpi and PBS-inoculated hamsters killed at 26 wpi. Three animals were examined for each time point. The horizontal dotted line indicates the 27% calculated ThT fluorescence threshold based on the means of negative controls plus 4 standard deviations. e Skin samples from back area of sCJDMM1-inoculated Tg40h mice at 4, 12, 16, 20, 22, and 24 wpi and PBS-inoculated control mice were subjected to RT-QuIC assay with recombinant human PrP90-231 as the substrate. The RT-QuIC trace showed is representative of three-independent experiments with three different animals at each time point