Fig. 7.
PrPSc in PBS-inoculated hamsters co-habitated with 263K-inoculated hamsters. Representative western blotting of PrPSc amplified by sPMCA with brain homogenates from PBS-inoculated hamsters in cohabitation with 263K-inoculated hamsters killed at 4 a, 7 b, 10 c wpi from three-independent experiments. d Representative western blot analysis of PrPSc amplified by six rounds of sPMCA from back skin samples from the PBS-inoculated with PBS killed after 3, 4, 7, 10, and 11 weeks of cohabitation with 263K-inoculated hamsters from three-independent experiments. P-rounds: number of sPMCA rounds. All samples were treated with PK at 100 µg/ml except for the sample in the first lane of each blot that was used as a non-PK treatment control. The molecular weight markers are shown in kDa on the left side of each blot. All blots were probed with the 3F4 antibody. e Representative of RT-QuIC trace of back skin samples from PBS-inoculated hamsters co-habitated with the 263K-inoculated mice at different time points