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. 2019 Jan 16;10:232. doi: 10.1038/s41467-018-08175-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Mechanical analysis of a pNIPAM block fabricated by 3D laser lithography. a Optical micrograph in the AFM with overlayed indications for the force measurements and the line-scan. Scale bar is 50 µm. b Measured Young’s Modulus as a function of temperature for a stepwise heating and cooling of the sample. c Height measurement via line-scanning from the glass substrate on top of the pNIPAM block. The different colors depict several cycles of heating and cooling