Fig. 1.

Photomicrographs showing the whole macula of saccule after 48 h treatment (A) without (control) and with cisplatin (Cis) at concentrations of 10–1000 μM (B–F). Specimens stained with Alexa 488-conjugated phalloidin which preferentially labels the stereocilia bundles and cuticular plate of vestibular hair cells. (A) Note high density of hair cell stereocilia in specimen cultured without cisplatin for 48 h. (B–D) Many hair cells were missing after treatment with 10, 50, or 100 μM cisplatin for 48 h. (E–F) Hair cell density in cultures treated with 400 or 1000 μM cisplatin were greater than with 10 or 50 μM cisplatin.