Fig. 2.

Representative photomicrographs of vestibular explants from utricle, saccule and crista cultured for 48 h without cisplatin (Cis) or with 10, 50, 100, 400 or 1000 μM cisplatin. Specimens stained with Alexa 488-conjugated phalloidin which labels the hair cell stereocilia bundles. Macula of utricle (A1), macula of saccule (B1) and ampulla of crista (C1) cultured with 0 μM cisplatin for 48 h; note high density of stereocilia bundles in control cultures. Density of hair cell stereocilia reduced at cisplatin concentrations of 10–50 μM in utricle (A2-3), saccule (B2-3) and crista (C2-3), but hair cell density gradually increased as the concentration increased from 100 to 1000 μM (A4,5,6; B4,5,6; C4,5,6). D–F: Histograms showing mean (n = 6, +/−) number of hair cells per 0.22 μm² in utricle, saccule and ampula. Horizontal lines with p values above indicate significant differences between conditions (p < 0.05, 0.01 or 0.001, one-way ANOVA, Bonferroni post-hoc comparisons).